miniSIA-2 analyzer with Chem-on-Valve manifold


The Global FIA miniSIA-2 combines the power of FIA and SIA in a single instrument without need for any hardware changes. The concept was first described by Ruzicka in the FIA Tutorial

The system is equipped with two milliGAT pumps coupled to twin heated holding coils.  The tubing from these two holding coils merge at the center of a Chem-on-Valve™ (COV) monolithic manifold. This configuration allows the simultaneous merging of two streams which is a characteristic feature of FIA and a necessity for many chemiluminescence reactions.

An extra measure of modularity is built into the COV by making the detector flow cell a bolt-on piece.  This means that the same COV manifold can be configured with a short or long path length absorbance cell, fluorescence cell,  chemiluminescence cell, one of a host of different electrochemical cells, as well as cells that can be used together with bead detection or the increasingly powerful sequential injection chromatography (SI-Chrom) modes of operation.

miniSIA-2 can be configured as a Single line (A) system for SIA or two line system (B) for either SIA or FIA mode. The standard 10mm uv/vis absorbance cell uses fiber optic cables to pipe light to and from the flow cell.  A white LED and a W-Xe lamp are provided as light sources.  The spectrometer is an Ocean Optics USB-4000. Discuss other flow cell options with Global FIA.

miniSIA-2 mani

The sample port has an inlet and outlet which meet on the face of the COV rotor.  Thus the sample can be primed to the valve without drawing sample into the rest of the manifold where it can cause carryover.  The sample port can also be primed with the next sample while the present measurement is underway by using the second milliGAT pump (A) or miniaturized metering pump.


Power supply voltage

100 to 240 VAC, 50/60 Hz

Power consumption

100-240 VAC, 10A (max)

Sample size

10-50 µL

Heater temperature range

Ambient to 120 °C 

Light source

White LED and W-Xe lamp

Spectrometer wavelength range

350 to 1050 nm



Available Chemistries

PO4, NOx,NH3,SO4,SiO4,Fe, Mn, Al, Zn, Enzymatic assays

Sequence time

30-500 seconds

Precision mid calibration range 


Linearity of calibration curves



7.5 kg


20 x 30 x 25 cm (W x D x H)

Ambient operating temperature

4 to 60 °C 

Ambient operating humidity

25% to 85% non-condensing

Storage temperature

-25 to 65 °C non-condensing and non-icing


USB 2.0 and Wi-Fi 802.11ac



COV-FL1Fluorescence flow cell with LED excitation

COV-CLChemiluminescence flow cell

PC-P25232Photon counter


SIA-KITSIA Fittings kit

COV-LPFC10-HHeated long path length photometric flow cell, 10 cm



Brochure: pdfminiSIA-2 brochure.pdf630.19 KB

Manual: pdfman_miniSIA-2_-.pdf1.91 MB

Manual FloZF: pdfman_FloZF_5.2.pdf3.16 MB

Manual FloZF Getting Started: pdfman_FloZF_Getting_Started_-.pdf1.16 MB


Useful links

Two Line SIA Systems

Simultaneous Nitrite & Nitrate Assay

Simultaneous Assay of P and Si